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KMID : 0380020050200010040
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 1 p.40 ~ p.45
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Effects of the Spatholobi caulis extract on Antioxidation and Inhibition of Matrix Metalloproteinase in Human Skin Fibroblasts
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Sim Gwan-Sub
Kim Jin-Hwa
Lee Dong-Hwan
Park Seong-Min
Pyo Hyeong-Bae
Lee Beom-Cheon
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Abstract
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The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of exracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In this study, to investigate the relationship between aging and Spatholobi caulis extract, we examined the effects of antioxidant, in vitro MMP inhibition and expression of UVA-induced MMP-1 in human dermal fibroblasts. Spathoiobi caulis extract was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the values of against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Spatholobi caulis extract inhibited the activities of MMP-1 in a does-dependent manner and the IC50 value calculated from semi-log plots was . Also, UVA induced MMP expression was reduced by treatment with Spatholobi caulis extract, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore Spatholobi caulis extract was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that Spatholobi caulis extract may act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.
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KEYWORD
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Spatholobi caulis, 1, 1-diphenyl-2-picrylhydrazyl, radical scavenging activity, matrix metalloproteinase, human skin fibroblast
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